IPSC-DYR0100-CAS9 cell line

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location： Home > Products > Cas9 Stable Cell Lines > IPSC-DYR0100-CAS9

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IPSC-DYR0100-CAS9 Order

IPSC-DYR0100-CAS9 cell line
Catalog#
YC-C098-Cas9-H
Size
1*10^6
Transfection Culture Medium
EZ-Stem™ Cell Culture Medium
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
IPSC-DYR0100-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
IPSC-DYR0100-CAS9
Morphology
Spherical clone
Passage ratio
1:6-1:8
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
EZ-Stem™ Cell Culture Medium
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the IPSC-DYR0100 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a IPSC-DYR0100-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data（RT-QPCR）
Sample Name Target Name Cт Mean ΔCт
IPS-CAS9 Cas9 21.07497406 7.93462086
IPS-CAS9 h-actin-B 13.14035320
IPSC-DYR0100 Cas9 34.49109268 21.41424656
IPSC-DYR0100 h-actin-B 13.07684612
Cutting Efficiency Validation
IPSC-DYR0100-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the IPS-CAS9 stable cell pool which is electroporated by ABCA gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:IPSC-DYR0100		 STR Info (Cell bank)
Cell Line:ATCC- DYR0100 hiPSC
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 11131113
D7S820 910910
D16S539 12121212
VWA 14191419
TH01 9.39.39.39.3
AMEL XYXY
TPOX 811811
CSF1PO 711711
D12S391 1819.3
FGA 2122
D2S1338 1719
D21S11 3032.2
D18S51 1013
D8S1179 1415
D3S1358 1517
D6S1043 1213
PENTAE 77
D19S433 1415
PENTAD 1213
D1S1656 1217.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
IPSC-DYR0100-CAS9 cell line
IPSC-DYR0100-CAS9
Catalog#
YC-C098-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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